The 3rd International Conference on Signal Processing and Machine Learning (CONF-SPML 2023) was held online on February 25, 2023. The accepted papers of CONF-SPML 2023 will be published in IOP Journal of Physics: Conference Series (Print ISSN: 1742-6588) or Applied and Computational Engineering (Print ISSN: 2755-2721). Prof. Roman Bauer from the University of Surrey, Prof. Brian A. Barsky from the University of California, Prof. Festus Adedoyin from Bournemouth University, Prof. Arshad Hassan Khan from the Department of Electrical Engineering NUCES-FAST, Islamabad, and Prof. Mazhar Javed Awan from University of Management & Technology have given keynote speeches on related topics of computational modelling, machine learning, electronic biosensor, etc. Also, we invited authors of qualified papers to deliver oral presentations at the online session. They have presented their studies of blockchain-based service network and virtual technology. Questions from the audience were collected and answered by the presenters. The conference provided the participants with good opportunities to exchange ideas and build networks, and it will lead to further collaborations between both universities and other societies.